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KMID : 1098420070150040285
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 4 p.285 ~ p.290
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Agrobacterium-mediated Transformation of PAT into Platycodon grandiflorum A. De. candolle
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Yun Jong-Sun
Yang Deok-Chun
Lee Cheol-Hee
Yun Tae
Kim Ik-Hwan
Park Jae-Seong
Hong Eui-Yon
Jeong Jae-Hun
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Abstract
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This study was conducted to introduce phosphinothricin acetyl transferase (PAT) gene, resistant to basta which was non-selective herbicide, into balloon flower (Platycodon grandiflorum A. De. candolle). Seeds were germinated on MS medium, and 10-day-old immature cotyledon explants and 30-day-old leaf explants were cocultured with Agrobacterium tumefaciens strain MP 90 (pBinSyn) on 1/10 MS medium for 48 hours in the dark at . The cultures were transferred for selection of kanamycin-resistant shoots to the MS medium supplemented with 0.2 NAA, 1.0 BA, 3% sucrose, 100 kanamycin, 500 carbenicillin. Shoots were obtained from 10-day-old immature cotyledon explants after 4 weeks of culture. The shoots were subcultured twice every 4 weeks on the same medium for growth of transgenic shoots. Successful transformation was confirmed by histochemical GUS assay, PCR analysis, RT-PCR analysis, 10 phosphinothricin treatment and 0.3% basta spray. The basta-resistant transgenic plants flowered normally.
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KEYWORD
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Platycodon grandiflorum, Agrobacterium, PAT, transformation
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